keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
200 mg | In stock | $ 48.00 | |
500 mg | In stock | $ 70.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 54.00 |
Description | Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes. |
In vitro |
METHODS: Mouse PDAC cells 14387T were transfected with lentiCRISPRv2 hygro lentivirus, and after 48 h, the transfected cells were cultured in new medium containing Hygromycin B (500 μg/mL) for two weeks, and the successful transfected cells were screened. RESULTS: Puromycin dihydrochloride screened the lentivirally successfully transfected cells. [1] METHODS: E. coli was cultured in medium containing Hygromycin B (0-8 μg/mL) and cell growth was monitored using a Klett-Summerson colorimeter. RESULTS: Hygromycin B reduced the number of viable cells and increased doubling time in a concentration-dependent manner.Hygromycin B inhibited the number of viable cells by 50% at a concentration of 20 μg/mL, and halved the rate of growth at a concentration of 25 μg/mL. [2] |
In vivo |
METHODS: To assay antiviral activity in vivo, Hygromycin B (0-5 μmoL/kg) was administered intraperitoneally to MHV-A59-infected BALB/c mice twice daily for three days. RESULTS: Hygromycin B was able to reduce the levels of viral replication and necrotic liver foci in vivo. [3] |
Synonyms | Hygrovetine |
Molecular Weight | 527.52 |
Formula | C20H37N3O13 |
CAS No. | 31282-04-9 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (56.87 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Hygromycin B 31282-04-9 Microbiology/Virology ribosome Antibacterial Antibiotic Antifungal inhibit Fungal Bacterial Hygrovetine Inhibitor inhibitor